Construction of a novel taste sensor using the taste receptor protein of vertebrate origin
Construction of a novel taste sensor using the taste receptor protein of vertebrate origin
批准号:
20688015
负责人:
MISAKA Takumi
金额:
$16.31万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (A)
财政年份:
2008
资助国家:
日本
项目状态:
已结题
起止时间:
2008 至 2009
中文摘要
我们计划利用味觉受体蛋白构建一种新的味觉传感器。味觉审查员应该给品尝者一个稳定的分数。为了测量甜度,我们产生了一个稳定表达人类甜味受体(hT1R2/hT1R3)的细胞系。该细胞可作为甜味传感器,获取细胞内钙指示染料荧光强度的变化。
英文摘要
We planned to construct a novel taste sensor using the taste receptor protein. The taste censor should be present a stable score to the tastants. To measure the sweetness, we generated a cell line that were stably expressing the human sweet taste receptor (hT1R2/hT1R3). The cell could be worked as a sensor for sweet taste to obtain the change of fluorescent intensities of the intracellular calcium indicatordye.
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Amiloride reduces the sweet taste intensity by inhibiting the human sweet taste receptor Biochem.
阿米洛利通过抑制人类甜味受体 Biochem 来降低甜味强度。
DOI:
--
发表时间:
2010
期刊:
Biophys. Res. Commun. (in press)
影响因子:
--
作者:
[Imada, T., Misaka, T., Fujiwara, S., Okada, S., Fukuda, Y., Abe, K.]
通讯作者:
K.
DOI:
10.1016/j.bbrc.2009.05.069
发表时间:
2009-07-31
期刊:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
影响因子:
3.1
作者:
[Ishii, Sho, Misaka, Takumi, Abe, Keiko]
通讯作者:
Abe, Keiko
甘味受容体発現コンストラクト、これを発現させた細胞体、及びその利用
甜味受体表达构建体、表达其的细胞体及其用途
DOI:
--
发表时间:
2009
期刊:
影响因子:
--
作者:
[]
通讯作者:
pH-Dependent structural change in neoculin with special reference to its taste-modifying activity
Neoculin 的 pH 依赖性结构变化,特别是其味道调节活性
DOI:
--
发表时间:
2009
期刊:
Biosci.Biotechnol.Biochem 73
影响因子:
--
作者:
[Morita Y, Nakajima K, Iizuka K, Terada T, Shimizu-Ibuka A, Ito K, Koizumi A, Asakura T, Misaka T, Abe K]
通讯作者:
Abe K
ヒト甘味受容体安定発現細胞株の作製の試み
尝试创建稳定表达人类甜味受体的细胞系
DOI:
--
发表时间:
2009
期刊:
影响因子:
--
作者:
[今田隆将, 他]
通讯作者:
他
共 8 条
Characterization of chemical compounds contributing to the taste enhancer/suppressor using the taste receptor-expressing cells and examination of those molecular mechanisms
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批准号:16H04918
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$11.23万
-
财政年份:2016
-
负责人:MISAKA Takumi
-
依托单位:
Construction of a novel fluorescent measurement system to evaluate pH values in fluorescent imaging analysis
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批准号:15K14914
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项目类别:Grant-in-Aid for Challenging Exploratory Research
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资助金额:$2.5万
-
财政年份:2015
-
负责人:MISAKA Takumi
-
依托单位:
海外基金