Visualization of the site of phospholipid biosynthesis in Arabidopsis roots under low temperature conditions

Cct_2植株的CCT活性由高到低依次为WS、Cct_2、Cct_1 - 1和Cct_1 -1。Cct 1 - 1Cct 2幼苗在2. WS、cct 2和cct 1 -1幼苗根表皮细胞在室温下均表现出ER-Tracker荧光，在2 ℃和盐胁迫下表现出明显的膨大。相比之下，内膜结构的发展不太昂贵，在ct 1 -1 ct 2幼苗在环境温度和消失在寒冷或盐胁迫的幼苗。在转基因拟南芥根中，sGFP-CCT 1和sGFP-CCT 2荧光与ER-Tracker荧光相关。这些结果表明，功能性CCT是必需的胁迫诱导的ER发展和细胞伸长在拟南芥根。

WS, cct2, cct1-1and cct1-1 cct2 plants exhibited CCT activity in descending order. cct1-1 cct2 seedlings were unable to elongate roots at 2. Root epidermal cells showed ER-Tracker fluorescence comparably in WS, cct2 and cct1-1 seedlings at ambient temperature and expandedly at 2 or under salt stresses. In contrast, endomembrane structures developed less expensively in cct1-1 cct2 seedlings at ambient temperature and disappeared in cold-or salt-stressed seedlings. sGFP-CCT1 and sGFP-CCT2 fluorescence was associated with ER-Tracker fluorescence in transgenic Arabidopsis roots. These results suggest that functional CCTs are required for stress-induced ER development and cell elongation in Arabidopsis roots.