Structural and functional basis of the complex of mono-ADP-ribosylating toxin and substrate protein

为揭示C.产气荚膜杆菌iota毒素（Ia）和肌动蛋白，我们成功地结晶并解析了apo-Ia-肌动蛋白和apo-Ia的结构。通过与NADH-Ia和βTAD-Ia-actin复合物的结构比较，揭示了ADP-核糖基化后的结构变化。肉毒杆菌C3毒素ADP-核糖基化RhoA Asn 41。为了分析C3-RhoA复合物的结构，RhoA在E. coli，分别用His标签和GST标签获得稳定的RhoAF 25 N突变体。我们目前正在寻找该配合物的结晶条件。

To reveal the structural and functional mechanism of C. perfringens iota toxin(Ia) and actin, we successfully crystallized and solved the structure of the apo-Ia-actin and apo-Ia. We revealed the structural change upon the ADP-ribosylation by comparing the structures together with NADH-Ia and βTAD-Ia-actin complex.C. Botulinum C3 toxin ADP-ribosylates RhoA Asn41. For the structure analysis of C3-RhoA complex, RhoA was expressed in E. coli and the stable mutant of RhoAF25N was obtained using His-tag and GST-tag, independently. We are currently searching the crystallization condition for the complex.