Induction of hematopoietic stem cells from human pluripotent stem cells

我们尝试用非饲养层培养系统从人多能干细胞(ES细胞和iPS细胞)中诱导造血细胞。我们的分化方法包括两个步骤，首先形成球体，然后进行二次贴壁培养。我们通过低密度培养、无血清造血祖细胞诱导、多系造血联合红系诱导、无病毒安全的人iPS细胞、造血祖细胞来源的iPS细胞等方法，成功地获得了高效的中性粒细胞。

We tried to induce hematopoietic cells from human pluripotent stem cells(ES cells and iPS cells) using non-feeder culture system. Our differentiation methods consist of two steps with initial sphere formation followed by secondary adherent culture. We succeeded in efficient production of neutrophils by low cell density culture, serum-free culture for hematopoietic induction, multilineage hematopoiesis with concomitant erythroid induction, production of virus-free safe human iPS cells, and hematopoietic induction from human iPS cells derived from hematopoietic progenitor cells.