Functional analysis of glucocorticoid induced transcript 1(GLCCI1)

本研究的目的是鉴定合成糖皮质激素诱导的新基因GLCCI1的蛋白功能。我们发现GLCCI是一种由糖皮质激素-糖皮质激素受体复合体诱导的、大小约70 kDa的微管蛋白相关的磷酸蛋白。我们还发现，PAK1和动力蛋白轻链8都是GLCCI1的配基蛋白，GLCCI1干扰了PAK1的活性，抑制了LC8的生物发生，导致了T细胞的凋亡。我们的数据提示，GLCCI1在PAK1-LC8相互作用中的功能意义可能是一个新的下游级联，类似于合成的糖皮质激素作为免疫抑制剂的作用。

The aim of the present study was to identify the protein function of GLCCI1, a novel gene induced by synthetic glucocorticoid. We found that GLCCI was a tubulin-associated phosphoprotein migrated at ～70 kDa that was induced via glucocorticoid-glucocorticoid receptor complex. We also found that both PAK1 anddynein-light chain 8 were the ligand proteins of GLCCI1, and that GLCCI1 interfered the PAK1 kinase activity against LC8 biogenesis, which led to T cell apoptosis. Our data suggested that the functional implication of GLCCI1 in PAK1-LC8 interaction might be a novel downstream cascade resembling synthetic glucocorticoid action as an immunosuppressant.