Development of regenerative medicine for periapical bone defect

在大鼠下颌第一磨牙进行活髓切断术，然后将含有氢氧化钙的bFGF或VitapexR（VIT）应用于暴露的牙髓组织。bFGF组表达TGF-β1和BMP-2的细胞数量迅速增加，修复性牙本质形成时间早，数量多。结果表明，bFGF和维生素BAG处理的大鼠在细胞因子和生长因子表达的时间和程度上可能存在差异，bFGF和生物活性玻璃（BAG）联合应用可促进骨缺损的活跃新骨形成。BAG是一种具有良好骨传导性的生物材料。

Pulpotomy was performed in the mandibular first molars of rats, and then bFGF or VitapexR(VIT) containing calcium hydroxide were applied to the exposed pulp tissues. In the bFGF-treated rats, cells expressing TGF-β1 or BMP-2 rapidly increased in number, and newly formed reparative dentin was observed earlier and larger in amount than that seen in the VIT-treated rats. It is suggested that there may be a difference in the time and extent of expression of cytokine and growth factors between bFGF and VIT-treated rats.Active new bone formation was observed in the bone defect with a combination of bFGF and Bioactive glass(BAG). It is speculated that BAG was a useful biomaterial with high bone conductivity.