DNA-protein cross-links : Repair and chromosome damage induction

当细胞暴露于DNA损伤剂时，蛋白质通常共价捕获在DNA上，产生DNA-蛋白质交联（DPC）。然而，关于细胞如何减轻DPC的不利影响，我们知之甚少。在本研究中，我们开发了新的方法检测DPC。基因组DPC主要通过自发水解消除，切除修复在DPC的去除中没有发挥重要作用。这表明，DPC首先激活ATR DNA损伤反应途径，然后激活ATM途径。

Proteins are often covalently trapped on DNA, generating DNA-protein cross-links(DPCs), when cells are exposed to DNA-damaging agents. However, not much is known about how cells mitigate the adverse effects of DPCs. In the present study, we have developed novel methods for the detection of DPCs. Genomic DPCs were primarily eliminated by spontaneous hydrolysis, and excision repair played no significant role in the removal of DPCs. It was suggested that DPCs initially activated the ATR DNA-damage response pathway, followed by the ATM pathway.