Establishment of novel non-invasive test for differential diagnosis and severity evaluation of hepatobiliary diseases in dogs and cats.

(1)动态CT和使用新型造影剂SONAZ的CEU对肝细胞癌和其他恶性肿瘤的良恶性鉴别有一定的价值。这些无创性的影像诊断技术，尤其是SONAZOID-CEU，将成为未来兽医临床领域的重要工具。(2)9个肝纤维化相关基因的表达与犬肝纤维化程度呈显著正相关，表明其分子调控机制与人和小鼠相似。结果表明，使用肝组织样品的基因表达分析可以是一个有用的工具，用于评估犬肝纤维化的程度。(3)血液透明质酸（HA）浓度的测量与怀疑有肝病的狗可以是一个有用的诊断援助犬肝硬化。(4)超声测量胆囊射血分数是一种简便、实用的胆囊排空指标。本研究首次发现胆囊粘液囊肿犬和胆泥症犬均存在胆汁淤积。

(1) Both Dynamic-CT and CEU using new contrast agent SONAZOID are useful for distinguish benign and malignant, hapatocellular carcinoma and other malignant tumors. These non-invasive diagnostic imaging, especially SONAZOID-CEU will be important tools in veterinary clinical field in the future.(2) The expression of 9 fibrosis-related genes showed significant positive correlation with the degree of canine liver fibrosis, indicating the similar molecular regulation with human and mouse. Results suggest that the gene expression analysis using liver tissue sample could be a useful tool for evaluating the degree of fibrosis in dogs.(3) Measurement of the blood Blood hyaluronic acid(HA) concentration of dogs with suspected liver disease can be a useful diagnostic aid for canine cirrhosis.(4) Gallbladder ejection fraction estimated by ultrasonography was easy and useful as the index of gallbladder emptying. In this study, we firstly found that biliary stasis occurs not only in dogs with gallbladder mucocele but also in dogs with biliary sludge.