Microarray analysis to detect genes predicting organotropism of lung cancer metastasis.

肺癌容易转移到多个器官，如骨、肺、脑、肝和肾上腺。我们应用微阵列分析来寻找影响肺癌转移器官向性的基因表达。使用人肺腺癌细胞系。将细胞接种于裸鼠新鲜组织切片上进行培养。之后，从器官切片表面收集增殖的癌细胞，并再次在烧瓶中培养。然后获得每个器官的条件细胞。对这些细胞包括原始细胞进行微阵列分析，以检测每个器官中的特定基因改变。将从每个器官获得的条件细胞的基因表达与原始细胞进行比较。使用非配对t检验检查器官特异性改变。我们检测到76个基因为肾上腺转移特异性改变，22个基因与肺，212个与肝，25个与肾，141个与骨，和27个与肌肉。

Lung cancer easily metastasizes to multiple organs, such as bone, lung, brain, liver, and adrenal gland. We applied microarray analysis to find the gene expression influencing organotropism of lung cancer metastasis. Human lung adenocarcinoma cell lines were used. Cell were cultured on the fresh tissue slice of athymic mouse. After that, proliferated cancer cells were collected from the surface of organ slice and cultured in the flask again. Then the conditioned cells from each organ were obtained. Microarray analysis was applied to these cells including original cells in order to detect specific gene alteration in each organ. Gene expressions of conditioned cells obtained from each organ were compared with original cells. Organ specific alterations were checked using unpaired t-test. We detected 76 genes as adrenal metastasis specific alterations, 22 genes with lung, 212 with liver, 25 with kidney, 141 with bone, and 27 with muscle.