Investigation of the dielectrophoretic properties of parasitic bacteria in emulsion and construction of new separation and detection systems of bacteria

本文研究了乳状液中寄生菌的介电电泳特性，并研制了一种新的分离检测装置，以实现对乳状液中寄生菌的快速检测。通过对微池内电场分布、细菌介电电泳速度和运动轨迹的数值分析，得到了最佳的电极排列方式和分离条件。实际上，对各种细菌实现了选择性缩合。并采用介电电泳微滤膜与LCR仪相结合的方法对细菌数量进行了定量测定。

In the present study, the dielectrophoretic properties of parasitic bacteria in emulsion were investigated, and new separation and measurement apparatus was developed for rapid and simple detection. Optimum electrode arrangement and separation condition from oil droplet were obtained by numerical analyses of electric field distribution in microcell, and dielectrophoretic velocity and trajectory of bacteria. In actual, selective condensation was achieved for various kinds of bacteria. Moreover, the bacterial amount was estimated quantitatively by combination of dielectrophoretic micro filter and LCR meter.