Role of ESCRT system in triple negative breast cancer

由于乳腺癌通常影响相对年轻的女性，因此控制恶性表型的潜在机制具有重要意义。在这项研究中，我们研究了包括三阴性乳腺癌在内的乳腺癌是如何被EMT控制的，EMT是一种实体肿瘤改变形状并开始迁移的现象。一个决定蛋白质寿命的囊泡转运系统ESCRT被废除的乳腺癌细胞系，肿瘤在体内的增殖被减弱。根据侧群和CD44+/CD24^<-/低>判断，强迫表达twist或snail这两种诱导EMT的关键转录因子的细胞导致癌症干细胞增加。这些结果表明，ESCRT系统和EMT可能对乳腺癌的恶性表型有积极的控制作用。

Because breast cancer often affects relatively younger females,underlying mechanisms which control the malignant phenotype is of great significance. Inthis study, we investigated how breast cancers including the triple negative breast cancersare controlled by EMT, a phenomenon in which solid tumors change their shapes and startmigration. A breast cancer cell line in which ESCRT, a vesicular transport system that determines proteins lifetime, is abrogated, in vivo proliferation of tumors was attenuated. Cells with forced expression of either twist or snail, two key transcriptional factors which induce EMT, resulted in an increase in cancer stemness, as judged by side population and CD44+/CD24^<-/low>. These results suggest that ESCRT system and EMT may positively control the malignant phenotypes of breast cancers.