experience of myringoplasty using autologous fibrin membrane and platelet rich plasma.

简单的下膜成形术已经发展，使其更容易和安全进行。然而，这个过程需要由捐献的血液制成的纤维蛋白胶。此外，皮肤切口是必要的采取皮下结缔组织移植物。目前已有一些用胶原蛋白片或几丁质膜代替皮下结缔组织移植物的报道。然而，没有关于使用患者自身血液制成的膜进行鼓膜成形术的报道。本研究的目的是从安全的角度建立一种微创的使用患者自体血液的鼓膜成形术。我们开发了一种制备自体纤维蛋白膜和凝胶的方法。我们使用图像分析设备评估纤维蛋白膜和凝胶的组织学稳定性。即使在2周的评估中，纤维蛋白膜的微观变化也较少。2周时纤维蛋白膜纤维密度与筋膜密度比较，差异无统计学意义。有人认为，纤维蛋白膜可能为鼓膜的再生提供基础，以替代皮下结缔组织或筋膜。这些结果表明，使用纤维蛋白膜的鼓膜成形术可能是一种更安全、侵入性更小的手术。

The simple underlay myringoplasty has evolved, enabling it to be performed more easily and safely. However, this procedure needs fibrin glue that is made from donated blood. Furthermore, a skin incision is necessary to take the subcutaneous connective tissue graft. There are some reports regarding the methods of using a collagen sheet or chitinous substance membrane as a substitute for the subcutaneous connective tissue graft. However, no report has appeared regarding a myringoplasty using a membrane made from the patient's own blood. The aim of this study is to establish the procedure of less invasive myringoplasty from the aspect of safety using autologous blood from the patient.We have developed a procedure to make the autologous fibrin membrane and gel. We evaluated the histological stability of the fibrin membrane and gel using image analysis equipment. The fibrin membrane had less microscopic changes even at the 2-weeks assessment. There was no statistically significant difference between the density of the fibrin membrane fiber and the fascia at 2 weeks.It is thought that the fibrin membrane might produce a base for the regeneration of the tympanic membrane as a substitue for the subcutaneous connective tissue or fascia. These results indicates that myringoplasty using the fibrin membrane could well be a safer and less invasive procedure.