Integrative omics study on bone metastatic tumors using clinical samples for clinical applications

为了揭示转移性骨肿瘤的分子背景，我们建立了原发性肿瘤组织和骨转移组织的表达谱，这些组织是在手术时获得和储存的。本研究包括肺癌、乳腺癌、肝细胞癌、结直肠癌、胰腺癌、胃癌、肾细胞癌和腮腺恶性肿瘤。通过二维差异凝胶电泳和PROTOMAP建立蛋白质表达谱。通过微阵列检查总体microRNA表达。我们鉴定了与原发肿瘤相比，骨转移肿瘤中表达水平异常调节的基因。通过体外实验验证了所鉴定基因的功能意义。

To reveal the molecular backgrounds of metastatic bone tumor, we created the expression profiles of primary tumor tissues and bone metastatic tissues, which were obtained and storaged at the time of surgery. Lung cancers, breast cancers, hepatocellular carcinomas, colorectal cancers, pancreatic cancers, gastric cancers, renal cell carcinomas, and parotid glad tumors were included this study. Protein expression profiles were created by two-dimensional difference gel electrophoresis, and PROTOMAP. Global microRNA expression was examined by the microarray. We identified genes whose expression levels were aberrantly regulated in the bone-metastatic tumors compared with the primary tumors. The functional significances of the identified genes were verified by the in vitro experiments.