Characterization of a novel protein which inhibits adipocytes differentiation

已知哺乳动物中存在两种脂肪组织;白色和棕色脂肪组织。白色脂肪组织的生理作用是以脂肪的形式储存生物能量。与白色脂肪组织不同，棕色脂肪组织的作用是以热量的形式耗散能量，从而防止哺乳动物过量储存能量。我们获得了一个编码一种新蛋白的cDNA，该蛋白在棕色脂肪组织中大量表达。推测该蛋白C端的SH 3结构域可与dynamin结合，推测该蛋白与dynamin的结合可抑制内吞过程。当这种蛋白质在前脂肪细胞中过表达时，向成熟脂肪细胞的分化受到抑制。这些结果表明，一种新的蛋白质在棕色脂肪组织中大量表达，调节发动蛋白介导的内吞作用，其过表达抑制脂肪细胞分化。

Two kinds of adipose tissues are known to exist in mammals; white and brown adipose tissue. The physiological role of white adipose tissue is storage of bioenergy in the form of fat. Unlike white adipose tissue, the role of brown adipose tissue is dissipation of energy as heat, which prevents mammals from excess storage of energy. We obtained a cDNA encoding a novel protein which abundantly expressed in brown adipose tissue. The putative SH3 domain located on the C-terminal region of this protein can bind dynamin, and it is suggested that the binding of this protein to dynamininhibits endocytosis process. When this protein was overexpressed in preadipocytes, differentiation to mature adipocytes was inhibited. These results suggest that a novel protein abundantly expressed in brown adipose tissue regulates dynamin-mediated endocytosis and its overexpression inhibits adipocytes differentiation.