Physiological role of calcium transport system regulated by phosphatidylinositol

为了阐明NCX1和TRPC3在心血管疾病(高血压、动脉硬化)中的作用，我们利用平滑肌α-肌动蛋白启动子过表达野生型和显性阴性的TRPC3，获得了TRPC3转基因(TG)小鼠。利用这些小鼠，我们发现NCX1和TRPC3可能在α1肾上腺素受体激动剂诱导的血管反应和血压调节中发挥重要作用。此外，我们还发现，与野生型小鼠相比，NCX1+/-小鼠一侧后肢缺血后的血液恢复明显增强，表明NCX1参与了缺血诱导的血管生成。

To clarify the role of NCX1 and TRPC3 in cardiovascular diseases (hypertension, arteriosclerosis), we generated TRPC3-transgenic (Tg) mice by overexpressing the wild-type and dominant negative TRPC3 using the smooth muscle α-actin promoter. Using these mice, we showed that NCX1 and TRPC3 may predominantly play an important role in α 1-adrenoreceptor agonist-induced vascular responses and blood pressure regulation. Furthermore, we found that blood recovery after unilateral hindlimb ischemia was significantly augmented in NCX1+/- mice compared with that in wild-type mice, suggesting that NCX1 is involved in ischemia-induced angiogenesis.