Regulation of cancer stem cells by MDMX/MDM2 oncoproteins

建立了结肠癌干细胞体外培养体系。使用该系统，我们对p53通路和相关的癌蛋白Mdm2和Mdmx进行了生物学和生化分析。我们发现p53的靶基因p21在肿瘤干细胞分化过程中被诱导。我们进一步发现了Akt通路、HGF-c-Met通路和糖酵解酶的激活。这些数据表明，结肠癌干细胞的调控涉及到致癌途径的动态调控。

We have established in vitro system for cultivation of colon cancer stem cells. Using this system, we have performed biological and biochemical analyses of the p53 pathway and the associated oncoproteins, Mdm2 and Mdmx. We have found that p21, a target gene of p53, is induced upon the differentiation of the cancer stem cells. Further we discovered the activation of the Akt pathway, the HGF-c-Met pathway and glycolytic enzymes. These data suggest that dynamic regulation of the oncogenic pathways are involved in regulation of colon cancer stem cells.