Efficient derivation of neural stem/progenitor cells from common marmoset embryonic stem cells for preclinical study of spinal cord injury.

本研究建立了从非人类灵长类动物、普通狨猴胚胎干细胞（ESCs）中高效提取多能神经干细胞/祖细胞（NS/PCs）的体外培养方法。这些绒猴胚胎干细胞衍生的神经球可以反复传代并依次产生神经元和胶质细胞，包括星形胶质细胞和少突胶质细胞。重要的是，绒猴esc衍生的NS/PCs可以产生功能性神经元，并且通过重复传代显示出与胎儿脑源性神经球更接近的表达谱。因此，该培养系统适用于获得供体细胞用于同种异体移植到狨猴模型中。

We established in vitro culture method to efficiently derive multipotent neural stem/progenitor cells(NS/PCs) from non-human primate, common marmoset embryonic stem cells(ESCs). These marmoset ESC-derived neurospheres could be passaged repeatedly and sequentially gave rise to neurons and glial cells, including both astrocytes and oligodendrocytes. Importantly, marmoset ESC-derived NS/PCs could generate functional neurons, and showed closer expression profiles to fetal brain-derived neurosphres by repeated passages. Thus, this culture system is applicable to derive donor cells for allograft transplantation into marmoset models.