Elucidating the molecular mechanisms of the centromere loading of CENP-A at G2 phase

在分裂酵母中，认为在G2期加入CENP-A可能起到挽救细胞死亡的作用。为了确定参与g2加载途径的因素，我们筛选了g2加载缺陷细胞的多拷贝抑制因子。分离出5个候选基因，其中1个具有RNA结合基序。缺乏这种RNA结合基序和ams2基因的双突变体表现出合成致死性。本研究提示该因子可能在G2期CENP-A的着丝粒沉积中起重要作用。

In fission yeast, it is thought that the incorporation of CENP-A at G2 phase may act as a rescue of the cell lethality. To identify factors that involved in the G2-loading pathway , we screened for multicopy suppressors of G2-loading deficient cells.Five candidate genes are isolated, and one of them has RNA binding motifs. A double mutant lacking both this RNA binding motif and ams2 genes exhibits synthetic lethality. In this study suggest that the factor may be important in centromere deposition of CENP-A at G2 phase.