Establishment of rapid detection system for intra-amniotic infection and drug susceptibility test by PCR using eukaryote-made Taq polymerase

我们建立了一种利用真核合成Taq聚合酶检测羊水中细菌、支原体、脲原体和真菌的新方法。应用该系统对86例早产儿羊水细菌感染进行了评价。令人惊讶的是，我们发现细菌性脲原体双重感染13例，脲原体感染5例，细菌感染13例，早产病例感染率占37.2%。在双重感染病例中，羊水IL-8非常高（平均：129.5ng/ml），慢性肺部疾病的发生率也非常高（38.5%），而非感染病例（5.6%）。双感染病例羊膜穿刺术至分娩的时间明显短于未感染病例。

We have established a novel method to detect bacterial, Mycoplasma, Ureaplasma and fungi in the amniotic fluid using eukaryote-made Taq polymerase. Using this system, we have evaluated the bacterial infection in amniotic fluid of 86 preterm labor cases. Surprisingly, we have detected double infection with bacterial Ureaplasma in 13 case, Ureaplasma infection cases in 5 cases, bacterial infection cases in 13 cases, and infection rate in preterm labor cases accounted for 37.2%. In double infection cases, amniotic fluid IL-8 was very high （mean: 129.5ng/ml), and frequency of chronic lung disease was also very high （38.5%) compared to those in non-infected cases （5.6%). The duration from the amniocentasis to delivery in double infection cases was significantly shorter than that in no infection cases.