Study on new source of volatile organoiodine compounds by isolation and culture of marine microorganisms

利用流式细胞仪对海洋微生物的分离培养方法进行了优化。对从天然水中生产卤代烃的细菌进行了调查。分离细菌并在25℃下培养，用动态顶空气相色谱/质谱法测定培养样品上气相中甲基卤化物(甲基氯、甲基溴、甲基碘)的浓度。对从微咸水中分离的细菌进行16S核糖体-核糖核酸(16S RRNA)基因序列分析。属于红杆菌或假单胞菌的五种遗传不同的细菌在培养数天内产生甲基氯、甲基溴和甲基碘。在分离的红杆菌(HKF-1)的培养中，观察到最高的卤化甲烷产量。水生细菌可能是海洋环境中甲基卤化物的新来源。

The isolation and culture method for marine microorganisms was optimized using a flow cytometer. Bacteria were investigated for the production of halocarbon from natural waters. Bacteria were isolated and incubated at 25ºC, and the concentrations of methyl halides (methyl chloride, methyl bromide, methyl iodide) in the gas phase above cultured samples were determined using dynamic headspace gas chromatography/mass spectrometry. Bacteria isolated from brackish water were identified by 16 Svedberg unit ribosomal-ribonucleic acid (16S rRNA) gene sequence analysis. Five genetically different strains of bacteria belonging to Erythrobacter or Pseudomonas produced methyl chloride, methyl bromide, and methyl iodide for several days in the culture. Maximum production rates for methyl halide were observed in the culture of isolated bacteria (HKF-1) belonging to Erythrobacter. Aquatic bacteria could be a new source of methyl halides in marine environments.