Molekulare Analyse von Eisen- und Zink-Aufnahme Systemen in Escherichia coli mit Schwerpunkt ZupT

ZupT蛋白是在原核生物中铁和锌转运蛋白的ZIP（Zrt和Irt相关蛋白）家族的第一个被描述的成员。ZIP样蛋白存在于生命的各个领域，包括人类，并且是铁，锰和锌稳态的重要因素。我们计划的目标是（i）了解ZupT在大肠杆菌中其他亚铁转运蛋白中的生理作用，（ii）鉴定ZupT中对功能和金属特异性重要的氨基酸残基，（iii）与Christopher Rensing合作，以生物化学方式表征ZupT介导的转运（University of Arizona，Tucson，USA）和（iv）采用DNA微阵列分析鉴定E.杆菌

The ZupT protein is the first described member of the ZIP (Zrt- and Irt-related protein) family of iron and zinc transportes in prokaryotes. ZIP-like proteins occur in all domains of life, including man, and are important factors for iron, manganese and zinc homeostasis. The goals of our proposal are (i) to understand the physiological role of ZupT amongst other ferrous transporters in Escherichia coli, (ii) to identify amino acid residues in ZupT important for function and metal specifity, (iii) to characterize ZupT mediated transport biochemically in cooperation with Christopher Rensing (University of Arizona, Tucson, USA) and (iv) to identify new iron transporters employing DNA-micro array analysis for E. coli.