Identification of substrate molecules on the host immune cells of bacterial proteases by using radioisotopes and mass spectrometry.

对健康志愿者外周血单个核细胞的中性粒细胞进行了细胞表面放射性碘标记和细菌蛋白水解酶处理。中性粒细胞非常脆弱，很容易坍塌，因此很难将细胞表面的蛋白质与胞浆中的蛋白质分开。因此，寻找适合中性粒细胞的放射性标记和蛋白降解的条件正在进行中。为了从凝胶中回收放射性蛋白质，用干净的冲头从夹着玻璃纸的干凝胶中冲出蛋白质斑点是令人满意的。为了照顾那些不使用放射性同位素的人，有必要防止真空泵排出的含有微量放射性的废气在实验室扩散。本研究鉴定了金黄色葡萄球菌对纤维蛋白原和胶原的新作用，这是S金黄色葡萄球菌具有凝血障碍和组织破坏的毒力活性的原因。

Neutrophils from peripheral blood mononuclear cells of healthy volunteers were subjected to cell-surface radioactive-iodine labeling and to bacterial protease treatment. Neutrophils were so fragile and easily collapsed that it was difficult to separate proteins on the cell surface from those in the cytosol. Therefore, searching the conditions suitable for the radiolabeling and proteolysis for the neutrophils are underway. To recover the radioactive proteins from the gels, it was satisfactory to punch out the protein spots from dried gels sandwiched by cellophane sheets by using clean punches. It was necessary to prevent the exhaust air with trace amount of radioactivity from the vacuum pumps from diffusing in the laboratory to take care those people who do not use radioisotopes.In this study, new effects of Staphylococcus aureus staphopains on fibrinogen and collagen were identified that account for the virulence activities of S aureus for impaired clotting and tissue destruction.