Functional crosstalk between ARF and Rab small GTPases

ARF家族的小GTP酶，除ARF 6外，主要定位于高尔基体，在那里它们触发包被载体囊泡的形成。我发现，同时消耗ARF 1/ARF 3或ARF 1/ARF 4诱导Rab 4和Rab 11阳性再循环内体的广泛列表。此外，ARF 1/ARF 3是从再循环内体到质膜的再循环途径所必需的。另一方面，ARF 1/ARF 4是TGN 38和甘露糖-6-磷酸受体从再循环内体逆行转运到trans-Golgi网络（TGN）所必需的，但不是再循环途径所必需的。确定不同的ARF组合如何将货物分类到不同的细胞器并调节相同隔室的单独运输过程仍然很重要。ARF可能与不同的效应蛋白一起在不同的膜微区沿着起作用。

Small GTPases of the ARF family, except for ARF6, mainly localize to the Golgi apparatus, where they trigger formation of coated carrier vesicles. I found that the simultaneous depletion of ARF1/ARF3 or ARF1/ARF4 induced extensive tabulation of Rab4- and Rab11-positive recycling endosomes. Moreover, ARF1/ARF3 are redundantly required for recycling pathway from the recycling endosomes to the plasma membrane. On the other hand, ARF1/ARF4 are redundantly required for the retrograde transport of TGN38 and mannose-6-phosphate receptor from recycling endosomes to the the trans-Golgi network (TGN), but was not required for the recycling pathway. It remains important to determine how distinct combinations of ARFs sort cargos targeted to distinct organelles and regulate separate transport processes on the same compartments. ARFs may act within distinct membrane microdomains along with distinct effector proteins.