Analysis of virus replication mechanism using minigenome or recombinant virus system

为了系统地寻找与人副流感病毒2型(HPIV2)V蛋白相互作用的宿主因子，我利用酵母双杂交系统，鉴定了一些蛋白。Tetherin是一种细胞膜蛋白，可以限制许多被包裹的病毒的萌发。我证明了Tetherin与V蛋白相互作用，并抑制hPIV2的生长。V蛋白通过与caspase-1的相互作用抑制caspase-1介导的IL-1β的成熟。此外，它还与接头相关蛋白复合体1(AP-1)相互作用，AP-1对hPIV2的顶端分选很重要。

To search for host factors interacting with human parainfluenza virus type 2 (hPIV2) V protein systematically, I used the yeast two-hybrid system, and identified some proteins. Tetherin is a cellular membrane protein that restricts budding of a number of enveloped viruses. I demonstrated that tetherin interacts with V protein, and inhibits hPIV2 growth. The V protein inhibits the caspase-1-mediated maturation of IL-1beta via its interaction with caspase-1. Moreover, it interacts with adaptor-related protein complex 1 (AP-1) that is important for apical sorting of hPIV2.