Assessment of plasma miRNA as biomarkers for various diseases

血浆miRNA对于鉴定坏死细胞非常有用，因为miRNA表达模式是细胞特异性的。基于这一概念，我们证实了血浆miR-499、miR-124、miR-210和miR-126以及miR-216 a和216 b分别可用作心肌梗死、脑梗死、充血性心力衰竭和急性胰腺炎的生物标志物。此外，平滑肌细胞、心肌细胞和骨骼肌细胞可以通过肌肉特异性miRNA（即miR-1、133 a、133 b、145和499）的表达模式来区分。

Plasma miRNAs are very useful to identify necrotizing cells, since miRNA expression patterns are cell-specific. Based on this concept, we confirmed that plasma miR-499, miR-124, miR-210 and miR-126, and miR-216a and 216b, were useful as biomarkers for miocardial infarction, cerebral infarction, congestive heart failure, and acute panceratitis, respectively. Moreover, smooth muscle cells, cardiomyocytes, and skeletal muscle cells could be discriminated by expression patterns of muscle specific miRNAs, namely miR-1, 133a, 133b, 145, and 499.