Therapeutic strategy for peritoneal dissemination of ovarian cancer using dynamic phototherapy and clofibric acid

本研究旨在探讨卵巢癌细胞胞内原卟啉IX(PpIX)含量变化的原因以及光动力疗法(PDT)对腹膜癌的影响。1)DISS细胞和MCAS细胞的PpIX含量分别为67.7和22.4微克/分升。2)β-丙氨酸合成酶、血红素加氧酶和胆绿素还原酶B在MCAS中的表达较DIS高3倍以上。3)5-氨基酮戊酸甲酯(MALA)-PDT治疗组大鼠的存活时间明显长于对照组，去大脑皮层手术(DS)-MALA-PDT组大鼠的存活时间明显长于DS组。这些结果表明，PpIX不会在血红素合成和分解活跃的细胞中积聚，甲基-丙氨酸-PDT在卵巢癌治疗中是有前景的。在PDT治疗中，细胞内PpIX的含量是很重要的。

This study aimed to investigate the reason why intracellular amount of protoporphyrin IX (PpIX) varies in ovarian cancer cells and the effect of Photodynamic therapy (PDT) on peritoneal carcinomatosa. 1) The amount of PpIX (microg/dl) was 67.7 in DISS cells and 22.4 in MCAS cells. 2) Expression of delta-ALA synthetic enzyme, heme oxygenase and biliverdin reductase B in MCAS increased more then 3 times compared with those in DISS. 3) The rats treated with a methyl ester of 5-aminolevulinic acid (Methyl-ALA)-PDT was significantly longer in survival than those of the control and debulking surgery (DS)-Methyl-ALA-PDT significantly prolonged survival compared with DS alone. These results suggest that PpIX is not accumulated in cells in which heme synthesis and resolution is active and that Methyl-ALA-PDT is promising in ovarian cancer treatment. The amount of PpIX in cells is important in PDT treatment.