Optogenetic approach of gene regulation

为了产生可光激活的Cre，我们制作了一系列LOV结构域与Cre之间的融合蛋白，并将其与lox-Stop-lox-GFP一起导入HEK细胞，通过GFP检测Cre的活性。然而，无论光照条件如何，我们都看到了GFP信号，这表明LOV结构域并不能有效地抑制Cre的活性。然而，在一些结构中，GFP比其他结构更暗。我们将进一步研究这些结构，看看是否可以通过添加额外的突变来改进它们。

In order to generate photoactivatable Cre, we made a series of fusion proteins between LOV domain and Cre and introduce into HEK cells along with lox-stop-lox-GFP, which allows detection of Cre activity by GFP. However, irrespective illumination, we saw GFP signal, indicating that LOV domain did not effectively suppress the activity of Cre. However, in some of constructs, GFP was dimmer than others. We will further pursue these constructs to see if we can improve them by adding additional mutations.