Development of a three dimensional stretchcell culture system with a self-assembling peptide scaffold

为了有效的组织再生，我们开发了一种能够机械细胞刺激的三维细胞培养系统。在一种新开发的自组装肽凝胶和拉伸室组成的系统中，通过拉伸刺激，提高了三维培养的小鼠骨骼肌细胞ERK磷酸化程度和细胞增殖率。这些结果表明，三维拉伸细胞培养系统能够将机械刺激传递到细胞，并控制其行为，如细胞增殖。

For effective tissue regeneration, we have developed a three dimensional cell culture system capable of mechanical cell stimulation. The degree of ERK phosphorylation and the cell proliferation ratio of three dimensionally cultured mouse skeletal muscle cells were improved by stretch stimulations in a system composed of newly developed self-assembling peptide gel and stretch chamber. These results demonstrated that the three dimensional stretch cell culture system was able to transmit mechanical stimulation to the cell and control its behaviors such as cell proliferation.