Analysis of archaeal viruses infecting hyperthermophilic archaea

古生菌是有别于真核生物和真细菌的主要生命领域。一些来自古生代的独特的酶被应用于生物技术。由于用于基因操作的有用酶通常来自病毒，古生菌病毒可能具有这些有用的酶。然而，感染古生菌的病毒很少，特别是感染高温古生菌的病毒几乎没有报道。在这里，感染嗜热古菌的病毒是从温度超过80摄氏度的温泉中收集的，那里是嗜热古菌居住的地方。在别府、玉川和洞爷湖收集了温度接近100摄氏度的温泉水。取水用0.22μm滤池过滤，去除杂质。用50 kDa滤膜浓缩水中的病毒。用酶解法和酚/氯仿抽提法提取纯化DNA。对纯化的DNA进行亚克隆，并测定DNA序列，分析其功能。

Archaea belongs to a major domain of life which is distinct from Eukaryota and Eubacteria. Some unique enzymes from Archaea are applied for biotechnology. Since useful enzymes for gene manipulation often derived from viruses, archaeal viruses may have these useful enzymes. However, only a few viruses infecting archaea are reported, especially those infecting hyperthermophilic archaea are scarcely reported. Here, viruses infecting hyperthermophilic archaea are collected from hot springs having temperatures of more than 80?C where hyperthermophilic archaea are living. Hot spring water having temperatures of near 100 ?C in Beppu, Atagawa and Toyako was collected. The obtained water was filtered with 0.22 μm filters and impurities were removed. Viruses in the water were concentrated with 50kDa-filters. DNA was extracted and purified with protease treatment and phenol/chloroform extraction. The purified DNA was subcloned and the DNA sequences were determined to analyze their function.