Development of novel affinity purification method using metal enolate

为了鉴定生物活性物质的结合蛋白和结合部位，我们计划开发一种新的亲和纯化方法，利用从羰基化合物和金属络合物中衍生出来的“金属烯醇”。S的研究结果表明，利用固载钯配合物，我们成功地捕捉到了水介质中含有羰基的氨基酸衍生物--钯的烯醇酸。此外，我们还成功地实现了含荧光基团的亲电试剂与钯的反应，从而实现了荧光标记。

To identify the binding proteins and binding sites of bioactive compounds, we planned ot develop novel affinity purification method using "metal enolate", which is derived from carbonyl compound and metal complex. As s result, by using solid-supported palladium complex, we successfully captured amino acid derivatives having carbonyl group as a palladium enolate in aqueous media. Moreover, we also succeeded in the reaction between electrophile having a fluorescent group and palladium enolate leading to fluorescent labeling.