Analysis of the diversity of fungal endophytes with metagenomics

利用ITS元条形码和Roche 454焦焦测序技术，对亚洲和澳大利亚2个低地热带森林和1个亚热带森林6种树种叶片内生真菌的α多样性和分类组成进行了研究。在97%的相似阈值下，共将95278个真菌reads聚类到224个otu中。在该门鉴定的178个otu中，子囊菌门和担子菌门的otu分别为148个和30个。主要OTUs为常见的叶面内生菌和病原菌。同一树种叶片间内生真菌组合相似性较低，表明具有较高的β多样性。

The alpha diversity and taxonomic composition of endophytic fungi associated with leaves of six tree species were examined in two lowland tropical forests and a subtropical forest in Asia and Australia, using ITS metabarcoding with Roche 454 pyrosequencing. A total of 95,278 fungal reads were clustered into 224 OTUs at 97% similarity threshold. Of the 178 OTUs identified to the phylum, 148 and 30 OTUs belonged to Ascomycota and Basidiomycota, respectively. Major OTUs were common foliar endophytes and pathogens. The low similarities of endophytic fungal assemblages between leaf samples of a single tree species suggested high beta diversity.