The role of phosphorylation status of radixin on the localization of billiary transporters

在这项研究中，我们研究了本地化的胆汁转运蛋白和生理性肝功能衰竭的慢性肝炎，通过使用人类肝脏切片。 我们可以通过Ethan DIGE二维荧光差异分析确定胆汁转运蛋白的新结合伴侣（AnexinA2等）。 我们可以表征AnexinA2负调控Mrp2和Bsep的小管膜定位。 此外，通过使用人夹心培养的肝细胞，在存在浓缩胆汁酸混合物的情况下，包括胆汁转运蛋白Bsep的抑制剂在内的抑癌药物（环孢菌素A和格列本脲）显示肝毒性。

In this study, we examined localization of biliary transporters and physiological liver failure in chronic hepatitis by using human liver slices. We could determine the new binding partners (AnexinA2 etc) of biliary transporters by using two-dimensional fluorescence differential analysis with Ethan DIGE. And we could characterize AnexinA2 which negatively regulates the canalicular membrane localization of Mrp2 and Bsep. Moreover, chlestatic drugs(CyclopsorinA and Glibenclamide), including inhibitors of Bsep, one of biliary transporter, shows hepatotoxicity in the presence of the concentrated bile acids mixtureby using human sandwich cultured hepatocytes.