Elucidation of the mechanism of VA-RNA-induced innate immune response

我们开发了一种VA-RNA-deleted Ad（Ad△VR）载体，其中va - rna表达的转录控制元件被删除。虽然传统的HEK293细胞不支持Ad△VR载体的繁殖，但通过适当诱导VA-RNA I的表达，诱导表达VA-RNA I的HEK293转化子（VR293细胞）允许Ad△VR载体的繁殖。Ad△VR载体在培养细胞中的转导效率与传统FG-Ad载体相当。Ad△VR矢量可能是FG-Ad矢量更安全的替代方案。

We have developed a VA-RNA-deleted Ad(Ad△VR)vector, in which the transcriptional control elements of the VA-RNA-expression were deleted. Although conventional HEK293 cells did not support the propagation of the Ad△VR vectors, HEK293 transformants inducibly expressing VA-RNA I (VR293 cells) with appropriate induction of VA-RNA I expression allowed the propagation of the Ad△VR vector. The Ad△VR vector showed high transduction efficiency comparable to that of the conventional FG-Ad vector in the cultured cells. The Ad△VR vector may be a safer alternative to the FG-Ad vector.