Mechanisms of mobilization of human multipotent mesoangioblast.

我们之前从接受心脏手术合并体外循环的患者中发现了循环成血管细胞（cmab），这是一种共表达KDR和Nkx2.5的间充质干细胞亚群。cmab能够分化为内皮细胞、平滑肌细胞和心肌细胞。最近，我们在动物模型中证明了肝细胞生长因子（HGF）诱导cMAB动员。我们假设肝素可以诱导人体内cMAB的动员，因为众所周知肝素可以非常迅速地上调血清中HGF的水平。我们发现肝素剂量依赖性地增加心导管患者血清HGF水平和cmab菌落数量。

We previously identified circulating mesoangioblasts (cMABs), a subset of mesenchymal stem cells that co-express KDR and Nkx2.5, from the patients undergoing cardiac surgery with cardiopulmonary bypass. cMABs are capable of differentiating into endothelial cells, smooth muscle cells and cardiomyocytes. More recently, we demonstrated that hepatocyte growth factor (HGF) induces cMAB mobilization in animal model. We hypothesized that heparin induces cMAB mobilization in humans because it is well known that heparin can very rapidly up-regulate the serum levels of HGF. We found that heparin dose-dependently increased serum HGF levels and the number of cMABcolonies in patients with cardiac catheterization.