A study of microglial function to cure Alzheimer disease

前列腺素E2降低培养的大鼠小胶质细胞的细胞活力。 这种细胞活力的降低是由于细胞凋亡。 两种PGE 2受体激动剂，EP 2激动剂布他前列素和EP 4激动剂PGE 1乙醇也诱导小胶质细胞凋亡。 然而，PGE 2降低的细胞活力不能被EP 1-EP 4拮抗剂逆转。 另一方面，GSK-3抑制剂SB 216763可逆转PGE 2降低的细胞活力。 此外，磷酸化GSK-3水平被PGE 2降低。 这些数据表明，PGE 2诱导小胶质细胞凋亡，这种凋亡参与GSK-3。

PGE2 reduced cell viability in cultured rat microglia. This reduction in cell viability was due to apoptosis. Microglial apoptosis was also induced by two PGE2 receptor agonists, an EP2 agonist butaprost and an EP4 agonist PGE1 alcohol. However, PGE2-reduced cell viability was not reversed by EP1 - EP4 antagonists. On the other hand, PGE2-reduced cell viability was reversed by GSK-3 inhibitor SB216763. In addition, phsophorylated GSK-3 level was reduced by PGE2. There data suggest that PGE2 induces apoptosis in microglia, and this apoptosis is involved in GSK-3.