A basic studyon malignant mesothelioma pathogenesis by internal radiation exposure of radium contained in the iron-containing protein

10只健康雄性Slc: Wistar大鼠反复注射螯合剂和铁。10只大鼠中有5只出现恶性腹膜间皮瘤，对照组和给药组均未出现恶性腹膜间皮瘤。肿瘤细胞Calretinin和CEA染色呈阳性。免疫染色及电镜示上皮性间皮瘤。8-羟基-2-脱氧鸟苷是DNA氧化损伤标志物，免疫染色阳性。因此，传统理论得到了证实。包括镭在内的微量元素的最终分析正在进行中。

Ten healthy male Slc: Wistar rats were repeatedly injected (i.p.) with a chelating agent and iron. Five of 10 rats showed a malignant peritoneal mesothelioma, but no rats showed it in the control and the chelating agent administration group. The tumor cells were positively stained by Calretinin and CEA. The results of immunostaining and the findings by electron microscope indicated epithelial mesothelioma. In addition, immunostaining with 8-hydroxy-2-deoxyguanosine is a DNA oxidative damage marker was positive. Thus, the conventional theoriy was confirmed. The final analysis of trace elements including radium is ongoing.