Identification of Lung Cancer Stem Cell Markers

通过使用临床标本，我们对可能的致癌细胞标志物进行了IHC分析。在这些标记中，CD166可能是最有用的。为了确定新的肺癌启动细胞标记物，我们分析了一个开放的数据库，筛选了在CD166阳性细胞中高表达的基因，测量了这些基因在临床标本中的表达水平，并进行了shRNA诱导的敲除实验。我们发现一个基因可能是一种新的肺癌启动细胞标记物，也是未来癌症治疗的靶点。

By using clinical specimens, we performed IHC analysis for the putative cancer-initiating cell markers. Among the markers, we found CD166 may be the mostuseful one. To identify novel lung cancer-initiating cell markers, we analyzed an open database, screened genes that were highly expressed in CD166-positive cells, measured expression levels of such genes in the clinical specimens, and performed shRNA-induced knocking-down experiments. We identified that a gene could be a novel lung cancer-initiating cell marker, as well as a future target for the cancer treatment.