Stem cell culture system with optimal spaces and anti-gravitational stimuli

我们报道了一种新的细胞培养反重力装置的发明，它可以颠倒传统培养皿在培养基中的位置。此外，我们还可以通过该设备在培养介质中旋转任何类型的3D支架。我们的假设是：如果我们系统地改变细胞引力的方向，我们最终可以检测到重力对细胞行为的影响。本文详细介绍了该反重力装置的结构和概念，以及该装置在成骨细胞MC-3T3 E1和癌细胞MG 63上的初步应用结果。结果显示，在反重力环境下，细胞形态发生了显著变化，肌动蛋白和胶原的RNA值增加了10~20倍，表明细胞骨架和蛋白质产物完全重排。

We report here a new invention of an anti-gravity device for cell culture, in that we can invert the position of conventional culture dishes up-side-down in the culture medium. Also, we can rotate the 3D scaffolds of any kind in culture medium by this device. Our hypothesis is: if we change the direction of gravitational force on cell systematically, we can eventually detect the effects of gravity on the behaviors of cells. In this paper we described the details of the structures and concept of anti-gravitational device, and also some of the preliminary results of application of the device for osteoblasts MC-3T3 E1 and cancer cells MG 63. Results included remarkable morphological changes of the cells under the anti-gravitational environment, with 10-20 times higher RNA values of actin and collagen, indicating total rearrangement of cyto-skeletons and protein productions.