Mechanisms of colon cancer stem cell self-renewal and pluripotency

癌症干细胞被认为是转移和癌症复发的原因。我们利用FACS分选技术从人结肠癌异种移植瘤中分离出肿瘤干细胞和非肿瘤干细胞，以分析基因表达。RNA-seq分析显示，374个基因在CSCs中显著上调。此外，我们发现，13个基因中的任何一个被敲除都会导致CSCs生长减少。此后，我们将研究这些基因在CSC行为中的重要性。

Cancer stem cells are thought to be responsible for both metastasis and cancer recurrence. We utilized FACS sorting to isolate cancer stem cells (CSCs) and non-cancer stem cells from xenograft human colon tumors to analyze gene expression. RNA-seq analysis revealed that 374 genes were significantly up-regulated in CSCs. Furthermore, we found that knockdown of any of 13 genes resulted in decreased growth ofCSCs. Hereafter, we will investigate the importance of these genes in CSC behavior.