Roles of laminin g2 chain in tumor growth and invasion.

为了分析LMG2链单体在体内对肿瘤生长和侵袭的影响，我们在人膀胱癌细胞系EJ-1和人胚胎肾细胞系HEK-293中过表达了G2链。当移植到裸鼠皮下或腹膜内时，过表达Gamma2的细胞生长速度更快，产生的实体瘤比对照转基因细胞大得多。这些数据有力地表明，G2单体促进了间质组织中肿瘤细胞的侵袭性生长。接下来，我们在HEK-293中过表达了三个不同的2标志，并分析了2链的活性部位。结果发现，2链Domein V(2dV)对肿瘤生长有强烈的诱导作用。在此基础上，我们建立了抗人层粘连蛋白2链V区的单抗。抗体的进一步详细特征正在进行中。

To analyze the effect of Lm g2 chain monomer on tumor growth and invasion in vivo, we overexpressed of the g2 chain in the human bladder carcinoma cell line EJ-1 and human embryonic kidney cell line HEK-293. When implanted into nude mice subcutaneously or intraperitoneally, gamma2 overexpressed cells grew faster and produced much larger solid tumors than the control transfectant. These data strongly suggest that the g2 monomer contribute to the invasive growth of tumor cells in stromal tissues. Next, we overexpressed three different 2 flagments in the HEK-293 and analyzed the active site of the 2 chain. It was found that domein V of 2 chain (2dV) strongly induced the tumor growth. Then, we established monoclonal antibodies against the domain V of human laminin 2 chain. Further detailed characterization of the antibody is in progress.