Creation of Novel Anti-allergic Leads Based on the Inhibition for Expression of IgE Receptors

过敏是由肥大细胞脱颗粒的化学介质引起的。这种脱颗粒是通过两个重要的步骤来实现的。在第一步中，将免疫球蛋白ES(IgE)附着在肥大细胞表面的IgE受体上。然后，肥大细胞上的IgE在第二步与入侵的过敏原反应。在这种情况下，我想知道肥大细胞上IgE受体的减少或许能够抑制过敏反应。基于这一思想，建立了免疫荧光染色和流式细胞术检测培养的肥大细胞表面IgE受体数量的检测体系。通过从药用植物中筛选出抑制IgE受体表达的抑制剂，发现氯素e6是一种有效的抑制剂。幸运的是，氯素e6不仅在体外，而且在大鼠体内实验中都抑制了IgE受体的表达。通过灌胃给药，明确了e6对大鼠体内过敏反应的抑制作用。

Allergy is caused by chemical mediators degranulated from mast cells. This degranulation is derived through the important two steps. In a first step, immunoglobulin Es (IgEs) are attached on the IgE receptors on the surface of mast cells. Then, the IgEs on mast cells react with invaded allergens in a second step. In this circumstance, I wondered that the decrease of IgE receptors on the mast cells may be able to suppress allergic reactions. Based on this idea, I established the assay system for evaluation of the amount of IgE receptors on the surface of cultured mast cells by immunofluorescence staining and flow cytometry. By the screening from medicinal plants for inhibitors against expression of IgE receptors, chlorin e6 was found as a potent inhibitor. Fortunately, chlorin e6 suppressed the expression of IgE receptors not only in vitro but also in vivo rat experiments. Finally, chlorin e6 was clarified to inhibit in vivo allergic reaction of rats through po administration.