Validation of the presence of myeloid-B progenitor stage towards to B cells on the differentiation pathway of hematopoiesis

我们提出了一种造血模型，在这种模型中，髓系潜能与向T、B和红系谱系的发育途径一起保留。在本项目中，我们旨在通过与基质细胞共培养系统验证骨髓-B祖细胞在多能祖细胞向B细胞转变过程中的存在。我们发现pre-pro B细胞仍然保留髓细胞潜能而几乎不保留T细胞潜能，而proB细胞终止向其他谱系的分化潜能。这些结果证实在向B细胞分化的途径上存在髓-B祖细胞阶段。这个项目已经成功完成。

We have proposed a model of hematopoiesis in which myeloid potential is retained along with the developmental pathway towards to T, B and erythroid lineages. In this project, we aimed to validate the presence of myeloid-B progenitors on the way from multipotent progenitors towards to B cells using co-culture system with stromal cells. We found that pre-pro B cells still retain the myeloid potential while hardly maintaining T cell potential, and that proB cells terminate the differentiation potential to other lineages. These results substantiate that the myeloid-B progenitor stage exist on the differentiation pathway towards to B cells. This project has been successfully achieved.