Development of a novel hydrogel for in situ Laser-Assisted Bioprinting to promote bone regeneration

制作明胶(Gel5wt%)-含有生物活性玻璃（BG0wt%, 1wt%， 3wt%或5wt%）的薄片，并进行脱水交联（CL120°C， CL140°C或CL160°C 24小时）。在单培养和共培养模型中，“Gel5wt% BG1wt% CL160°C”的条件下，在物理（孔隙度、孔径、膨胀比）、机械（杨氏模量）和生物（活力、增殖、ALP活性、钙沉积）性能方面获得了最好的结果。经模拟体液浸泡后，EDX测量的Ca/P摩尔比在1.72 ~ 1.77之间，表明该生物材料具有良好的生物活性。在共培养中，该配方支持微血管网络的形成。最后，这种新型生物纸成功地用作激光辅助生物打印技术的接收基板，但也适用于3D成型和堆叠方法来生成3D结构。

Gelatin (Gel5wt%)-sheets incorporating bioactive glasses (BG0wt%, 1wt%, 3wt% or 5wt%) were fabricated and underwent a dehydrothermal crosslinking (CL120°C, CL140°C or CL160°C 24h). The condition “Gel5wt% BG1wt% CL160°C” allowed to obtain the best results in terms of physical (porosity, pore size, swelling ratio), mechanical (Young modulus) and biological (viability, proliferation, ALP activity, calcium deposition) properties, in monoculture and coculture models. After immersion into simulated body fluid, Ca/P molar ratios measured using EDX analysis were between 1.72 and 1.77, suggesting a good bioactivity of the biomaterial. In coculture, this formulation supported the formation of microvascular networks. Finally, this novel biopaper was successfully used as a receiving substrate for Laser-Assisted Bioprinting technology, but also adapted to 3D-moulding and stacking approaches to generate 3D constructs.