A STUDY OF THE DISTRIBUTION OF AXONS CONTAINING SUBSTANCE P AND THE LOCALIZATION OF NEUROKININ-1 RECEPTOR IN TEMPOROMANDIBULAR JOINT ARTHRITIS OF THE RAT.

1. 免疫光镜及免疫电镜观察正常大鼠TMJ内nos的定位：6周龄雄性Wistar大鼠麻醉后经颅灌注固定剂，切除TMJ。脱钙后取冷冻切片，进行免疫光镜和免疫电镜观察。采用eNOS或iNOS抗体的ABC法。在面向TMJ腔上腔室的滑膜前部和后部以及面向下腔室的滑膜前部和外侧的滑膜衬里细胞中发现了eNOS和iNOS的免疫反应性。在靠近滑膜的血管内皮细胞中也检测到eNOS。免疫电镜观察显示，eNOS和iNOS的免疫反应性主要存在于滑膜衬里细胞的A型细胞中。在这些A型细胞中，eNOS和iNOS的免疫反应产物主要分布在细胞质中，以及囊泡和液泡结构中。免疫光镜下观察佐剂性关节炎大鼠TMJ内NOSs的定位：7周龄雄性Lewis大鼠注射佐剂诱导关节炎。免疫显微镜观察eNOS和iNOS的方法与上述方法相同。炎性TMJ滑膜衬里细胞中eNOS和iNOS的免疫反应性定位与正常关节相似。在面向上或下关节室的增生滑膜衬里细胞中发现eNOS或iNOS的免疫反应性。然而，eNOS的免疫反应强度比iNOS强。虽然正常大鼠和关节炎大鼠滑膜衬里细胞对iNOS的免疫反应性强度没有明显差异，但关节炎大鼠对iNOS的免疫反应性降低。

1. Immunolight- and immunoelectron-microscopic observation of the localization of NOSs in the normal rat TMJ : Six-week-old male Wistar rats were anesthetized and perfused transcardically with fixative, and TMJs were dissected out. After decalcification, frozen sections were obtained and submitted to immunolight- and immunoelectron-microscopic observation. ABC method with antibodies for eNOS or iNOS was utilized. Immunoreactivity for eNOS and iNOS was found in the synovial lining cells in the anterior and posterior portions of the synovium facing the upper compartment of the TMJ cavity, and in the anterior and lateral portions of the synovium facing of the lower compartment. eNOS was also detected in the endothelial cells of blood vessels close to the synovium. Immunoelectron-microscopic observation revealed that immunoreactivity for eNOS and iNOS was mainly found in the type A cells of the synovial lining cells. In these type A cells, immunoreactive products for eNOS and iNOS were distributed mainly in the cytoplasm, and in the vesicular and vacuolar structures.2. Immunolight-microscopic observation of the localization of NOSs in the rat TMJ with adjuvant-induced arthritis : Arthritis was induced by the injection of the adjuvant to 7-week-old male Lewis rats. immunolight-microscopic observation for eNOS and iNOS was performed in the same manner as the above mentioned method. The localization of immunoreactivity for eNOS and iNOS in the synovial lining cells of the inflamed TMJ was similar to that of the normal joint. Immunoreactivity for eNOS or iNOS was found in proliferated synovial lining cells facing the upper or lower joint compartment. The strength of immunoreactivity for eNOS was, however, stronger than that for iNOS.Although no clear difference was found in the strength of immunoreactivity for eNOS in the synovial lining cells between the normal rat and the arthritis rat, immunoreactivity for iNOS was reduced in the arthritis rat.