Comparative analysis of distinct seed dormancy mechanisms within the genus Lepidium (cress) and the evolution of the Brassicaceae DOG1 dormancy genes

拟南芥（Arabidopsis thaliana） DOG1 （DELAY OF GERMINATION1）是利用自然休眠遗传变异克隆到的首个种子休眠特异性基因。DOG1基因对拟南芥休眠至关重要，但其序列不能提供功能信息。我们希望通过野生型和突变型种子的比较定量分析来研究dog1相关的全种子蛋白质组。除了芸苔属的DOG1同源序列外，对其他物种的DOG1同源序列一无所知。我们从拟南芥种子较大的近亲Lepidium sativum中克隆了一个DOG1同源基因，将用于分析其种子组织特异性蛋白质组。衣草属（衣草）提供了一个单系进化枝，其物种在休眠（非休眠、被休眠或胚胎休眠的种子）方面有所不同。我们将研究不同休眠状态的韭草种子中DOG1的表达。我们将通过RNAi和意义转化改变它们的DOG1表达，研究对转基因Lepidium种子休眠的影响。

DOG1 (DELAY OF GERMINATION1) of Arabidopsis thaliana is the first seed dormancy-specific gene cloned by exploiting natural genetic variation in dormancy. The DOG1 gene is essential for Arabidopsis dormancy, but its sequence does not provide functional information. We want to investigate the DOG1-related whole-seed proteome by comparative quantitative analysis of wildtype and mutant seeds. Except for the sequence of a Brassica DOG1 homolog, nothing is known about DOG1 in other species. We have cloned a DOG1 homolog from Lepidium sativum, a close relative of A. thaliana with bigger seeds which will be used to analyse the seed-tissue-specific proteome. The genus Lepidium (cress) provides a monophyletic clade with species that differ in dormancy (non-, coat-, or embryo-dormant seeds). We will study DOG1 expression of Lepidium seeds with different dormancy. We will alter their DOG1 expression by RNAi and sense transformation and study the effect on dormancy of the transgenic Lepidium seeds.