Functional analysis of protein phosphatase in vestibulo-cereberum induced by unitateral labyrinthectomy in rats

采用差异显示法对单侧迷路切除术后小脑小叶中基因表达的改变进行了研究。从假手术大鼠和去迷路大鼠的絮凝体中分离总RNA，采用任意引物组进行PCR扩增，聚丙烯酰胺凝胶电泳分离。PCR产物的数量在迷路切除大鼠和对照大鼠的样品中有显著差异，从凝胶中取出并测序。其中一个上调的产物是大鼠蛋白磷酸酶2A β催化亚基mRNA，一个下调的产物是大鼠谷氨酸受体δ -2亚基mRNA。原位杂交的原位化学研究表明，这些分子定位于浦肯野细胞层。在去迷路大鼠中，UL诱导的眼球震颤在UL后3天内逐渐消失。这些发现表明，UL后这些分子在絮状浦肯野细胞中的表达变化参与了前庭代偿。

The differential desplay method was used to identify the gene expression which was altered in the cerebellar flocculus after unilateral labyrintyectomy (UL).Total RNA from flocculi of sham-operated and labyrinthectomized rats was isolated, amplified by PCR using arbitrary primer sets and separated by electrophoresis on a polyacrylarmide gel.PCR products, of which amounts were significantly different in samples from labyrinthectomized rats and those from controls, were cut out of the gel and sequenced.One of the up-regulated products was the rat protein phosphatase 2A beta catalytic subunit mRNA and one of the down-regulated products was the rat glutamate receptor delta-2 subunit mRNA.Ilistochemical study of in situ hybridization showed that those molecules were localized in the Purkinje cell layer.In labyrinthectomized rats, UL-induced nystagmus gradually disappeared within 3days after UL.These findings suggested that changes in expression of those molecules in the floccular Purkinje cells after UL is involved in vestibular compensation.