Involvement of limbic system and hypothalamus in bronchial allergic reaction

我们在以前的研究中报道了电解引起的双侧下丘脑前部损伤（阿勒）对豚鼠和大鼠气道速发型和迟发型变态反应的抑制作用，以及豚鼠条件性组胺释放的机制。本实验旨在研究微量注射红藻氨酸（Kainic acid，KA）代替电解产生的阿勒对大鼠气道过敏反应的抑制作用及其对下丘脑区神经细胞的激活作用，并探讨条件性组胺释放的脑机制。1)支气管肺泡灌洗液（BALF）和肺组织中的嗜酸性粒细胞（抗原激发后迟发性过敏反应的指标）在MPO组中均显著降低，MPO组在下丘脑的双侧内侧视前区注射红藻氨酸， ...更多信息 与非髓过氧化物酶组和假手术组比较。结果表明，用本实验方法制备的阿勒对大鼠气道迟发性变态反应有抑制作用，术后7 d，三组大鼠下丘脑室周区（PVH）、杏仁核等脑区神经细胞Fos蛋白表达无明显差异。本实验旨在观察致敏大鼠条件刺激后下丘脑c-fos mRNA的表达，以及从手术到脑组织固定的最佳时间。2）条件刺激后30 min，观察致敏大鼠条件组和非条件组脑神经细胞c-fos mRNA的表达。虽然c-fos mRNA在PVH中的表达较弱，但两组之间无显著差异。我们目前正在研究条件化的方法和原位杂交的方法。少

We have reported in previous studies the inhibition of the immediate and delayed type allergic reactions in the airway of guinea pigs and rats by the bilateral anterior hypothalamic lesioning (AHL) produced by electrolysis, and the mechanism of conditioned histamine release in guinea pigs. In this study, we carried out experiments in order to investigate whether the inhibition of allergic reaction in the rat's airway may or may not be caused by the AHL produced by microinjection of kainic acid instead of electotrlysis and the activation of the nerve cells in the hypothalamic area by the AHL, and to investigate the mechanism in the brain regarding conditioned histamine release.Results. 1) Both the eosinophils in bronchoalbeolar lavage fluids (BALF) and lung tissues, which are the indicators of the late allergic reaction after the antigen challenge, were significantly lower in the MPO group that was injected with kainic acid into the bilateral medial preoptic area of the hypothalamus by … More microinjection methods, when compared with those of the non-MPO group and sham-operation group. These results suggest that AHL produced by the method used in this study may inhibit the late allergic reaction in the airway of rats.There were no significant differences in the expression of Fos protein in the nerve cells at the periventricular hypothalamic area (PVH), amygdala and other areas in the brain 7 days after the operation among the three groups. We intend to investigate the expression of c-fos mRNA in the hypothalamic area after the antigen challenge and fhe optimal duration from the operation to the fixation of brain tissue.2) The c-fos mRNA expression in the brain nerve cells was investigated 30 minutes after the conditioned stimulus in both the conditioned and unconditioned groups of sensitized rats. Although a weak expression of c-fos mRNA in the PVH was observed, no significant difference was found between both groups. We are now investigating the methods of the conditioning and the protocols of in situ hybridization method. Less