Development of optical recording system for cardiac electrical activities and metabolism

本项目旨在开发一种新的光学记录系统，用于同步记录心脏准备中的电活动和代谢。该系统由4个主要部分组成：1）光学系统和记录室; 2）检测系统，具有多通道记录系统，用于电压敏感染料记录电活动; 3）第二检测系统，具有CCD摄像机，用于记录心肌细胞的代谢;制备室放置在显微镜的载物台上，其中构建了分束器，安装在隔振台上。显微镜上安装了两种光学检测系统：一种是16 × 16或12 × 12元件光电二极管阵列，用于检测与电活动相对应的外源性信号;另一种是冷却的CCD摄像机，用于检测反映心肌细胞代谢的NADH内源性荧光。使用这些探测器采集的数据通过LAN（局域网）传输到图形工作站，以直观地分析数据。在生理学实验中，使用从成年大鼠心脏解剖的右心房制备物。用部花青-若丹宁染料（NK 2761）对制备物进行染色，并使用含有2，3-丁二酮单肟的浴介质来减少收缩相关的光散射。利用该系统，分析了兴奋波的传导模式与心肌代谢状态之间的关系。

This project is aimed at developing a new optical recording system for the simultaneous recording of the electrical activity and the metabolism in the heart preparation. The system is composed of four main parts, including 1) optics and a recording chamber, 2) a detection system with a multiple channel recording system for the voltage-sensitive dye-recording of electrical activities, 3) the second detection system with a CCD camera for recording the metabolism of cardiac cells and 4) a computer for analyzing experimental data. The preparation chamber is placed on the stage of a microscope, in which a beam splitter is build, mounted on the vibration-isolation table. Two kinds of optical detection system are mounted to the microscope : a 16 X 16- or 12 X 12-element photodiode array for the extrinsic signals corresponding to the electrical activities, and a cooled CCD camera for the intrinsic fluorescence of NADH reflecting the metabolism of the cardiac cells. Data acquired using these detectors are transferred to a graphic workstation via LAN (local area network) to analyze the data visually. In the physiological experiments, right atrial preparations dissected from the adult rat heart are used. The preparations are stained with a merocyanine-rhodanine dye (NK2761), and a bathing medium containing 2,3-butanedione monoxime is used to reduce the contraction-related light scattering. Using this system, the relationship between the conduction pattern of the excitatory wave and the metabolic state of cardiac muscle is analyzed.