The Analysis of Gene Expression of Morphogenetic Factors Implicated Development of Tooth Germ and Odontogenic Tumors

牙齿形态学和细胞分化是由表皮-中间相互作用调节的，基础膜在调节这些相互作用的第四类聚合物中发挥作用，是牙科基础膜的主要组成部分，由三个α链组成。在cryosections上使用间接免疫荧光，我们在α1到α6型聚合物中的蛋白质本地化中表征了这些变化。We propose that the inner enamel epithlium in the cap stage is composed of type IV collagen consist of rap1，rap2 and rap4 chains，probally in molecular forms of rap1（IV）rap2（IV）rap4（IV）， [rap4（IV）]イイD23イD2 and [rap1（IV）]イD22イD2（IV）。这些表格包含α4链，但尚未在其他器官中检测到。因此，我认为这些形式与牙齿发展的形态发生和细胞分化特征有关，这些特征规范了基本-中间相互作用。最近，它已经被研究生化了，一些增长因素是由外延或中间组织绑定到IV型聚合物的更大亲和力、条件和贡献于有机形态发生在与基础薄膜的协作中。我们在牙齿凝胶开发期间观察了小鼠第一个摩尔摩尔生长因子和四类聚合之间的关系之间的关系（TGF-β、BMPs和FGFs）的几种生长因子的表达模式（TGF-β、BMPs和FGFs）。在Cap阶段和之后的地下室中没有检测到任何增长因素。在另一边，在牙科层和芽阶段，FGF-2和TGF-β1被检测到牙科层。FGF-2和TGF-β1可以在与分子形式合作的早期牙齿形态和细胞分化中发挥重要作用， α3（IV）α4（IV）α5（IV），以及在牙齿开发早期阶段的存在者。

Tooth morphogenesis and cytodifferentiations are regulated by epithelium-mesenchymal interactions, and the basement membrane plays a role in mediating these interactions Type IV collagen, the major component of the dental basement membrane, is composed of three αchains. Using indirect immunofluorescence on cryosections, we characterized the changes in protein localization of α1 to α6 chains of type IV collagen during mouse molar development. We propose that the inner enamel epithlium in the cap stage is composed of type IV collagen consist of α1, α2 and α4 chains, probably in molecular forms of α1(IV) α2(IV) α4(IV), [α4(IV)]ィイD23ィエD2 and [α1(IV)]ィイD22ィエD2 α2(IV). These forms containing α4 chain have not been detected in other organs yet. Therefore, it is thought that these forms is related to morphogenesis and cytodifferentiation characteristic of tooth development that regulated y epithelial-mesenchymal interactions.Recently, it has gradually been ascertained that some growth factors secreted by epithelial or mesenchymal tissue bind to type IV collagen with greater affinity, condens there and contribute to organ morphogenesis in cooperation with basement membrane. We observed the expression patterns of several growth factors (TGF-β, BMPs and FGFs) in the basement menbrane of mouse first molar during tooth germ development and explored the relation between these growth factors and the tooth germ-specific molecular forms of type IV collage. No examined growth factor was detected in the basement membrane in and after cap stage. On the other hand, in the dental lamina and bud stages FGF-2 and TGF-β1 were detected in the dental basement membrane. FGF-2 and TGF-β1 may play an important role in early tooth morphogenesis and cytodifferentiation in cooperation with the molecular form, α3(IV)α4(IV)α5(IV), which exsists in the early stage of tooth development.