Studies on the evaluation of health effects caused by toxic substances containing lead

本研究旨在评估铅和致癌性多环芳烃（PAHs）等有害物质对健康影响的个体差异。首先，我们研究了约200名职业铅暴露工人的δ -氨基乙酰丙酸脱水酶（ALAD）多态性与铅诱发的卟啉代谢紊乱的关系。以尿中δ -氨基乙酰丙酸（ALA）浓度和红细胞ALAD抑制率作为铅对健康影响的生物学指标。然而，我们没有发现ALAD酶多态性与铅暴露工人尿ALA浓度之间的显著关系。其次，我们研究了51名吸烟者和65名焦炉工人暴露于致癌物PAHs的淋巴细胞中细胞色素P450 （CYP1A1）和谷胱甘肽s-转移酶（GSTM1）等代谢酶的遗传多态性与DNA加合物水平的关系。DNA加合物的测量被认为是监测人类环境致癌物暴露的有用指标。这些数据表明，淋巴细胞中DNA加合物的水平不仅受多环芳烃暴露水平的影响，还受CYP1A1基因多态性的影响，表明Val/Val型CYP1A1的DNA加合物水平高于I1/I1和II/Val型CYP1A1。

We investigated to evaluate the individual differences of health effects caused by hazardous substances such as lead and carcinogenic polycyclic aromatic hydrocarbons (PAHs). First, we studied the relationship between polymorphism of delta-aminolevulinic acid dehydratase (ALAD) and lead-induced porphyrin metabolic disorders in about 200 workers occupationally exposed to lead. Urinary concentration of delta-aminolevulinic acid (ALA) and erythrocyte ALAD inhibition were used as biological parameters of health effect by lead. However, we could not find a significant relation between polymorphism of ALAD enzyme and urinary concentration of ALA in the lead-exposed workers.Secondly, we investigated the relationship between genetic polymorphism of metabolic enzymes such as cytochrome P450 (CYP1A1) and glutathione s-transferase (GSTM1)and DNA adducts levels in lymphocytes of 51 cigarette smokers and 65 coke oven workers who are exposed to carcinogen PAHs. The measurement of DNA adducts is considered a useful indicator for environmental carcinogen exposure monitoring in humans. The data obtained demonstrated that the level of DNA adducts in lymphocytes can be influenced by not only level of exposure to PAHs but also genetic polymorphism of CYP1A1, indicating that DNA adducts levels were higher in Val/Val type of CYP1A1 than in I1/I1 and II/Val types of CYP1A1.